Polylink protein coupling kit
WebFeb 18, 2013 · Intranasal administration is used for a wide variety of drugs, including proteins, ... PolyLink Protein Coupling Kit Product data sheet 644. 2010 Product Data Sheet 644, ... WebFeb 12, 2024 · Carboxyl polystyrene beads (CP-20–10, diameter 2.1 μm, Spherotech) were covalently coated with sheep anti-digoxigenin antibody (Roche) via carbodiimide reaction (PolyLink Protein coupling kit ...
Polylink protein coupling kit
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WebPolyLink - Protein Coupling Kit for COOH Microparticles For microparticles 1.0 micron or larger Kit Contents: 2435A - PolyLink Coupling Buffer (55ml): 50mM MES, pH 5.2, 0.05% … WebThe PolyLink Protein Coupling Kit contains buffers and carbodiimide (EDAC) derivative for coupling proteins to carboxylated beads. The contents of the kit are sufficient for 50 …
WebPL02N PolyLink Protein Coupling Kit with Vivaspin® - EDAC component 2435C 1.2 Relevant identified uses of substance or mixture and uses advised against Identified uses: Lab use 1.3 Details of the supplier of the safety data sheet Company: Bangs Laboratories / A Division of Polysciences WebJul 16, 2012 · For conjugation of FERM to 2-µm carboxylated polystyrene microspheres (Polysciences, Inc.), 1 mg of FERM was added to 12.5 mg of microspheres and coupled using the PolyLink protein coupling kit instructions (Polysciences, Inc.) with an additional block using 5% low–molecular weight dextran in 0.25 M glycine, pH 8.0.
WebSpecialist distributor of life science reagents in the UK: PolyLink Protein Coupling Kit for COOH Microspheres, Diameter (PL01N-1KIT) by Bangs Laboratories WebFluoresbrite microparticles and Polylink Protein Coupling kit for COOH microparticles were purchased from Polysciences, Inc. (Warrington, PA, USA). Antibodies: Abs specific for phospho-SHP-1, phospho-Syk and Akt were purchased from Cell Signaling Technology (Boston, MA, USA).
WebThe contents of the kit are sufficient for 50 coupling reactions using 200-500µg of protein per reaction. The kit has been optimized using purified IgG as the coupling protein. …
WebPolyLink Protein Coupling Kit 24350-1 for COOH Microsparticles tebu-bio is a European company specialised in providing innovative reagents and laboratory services in Life … small plate dinner ideasWebMar 18, 2024 · Immobilization of recombinant EF-Tu protein on microbeads. Recombinant EF-Tu protein was coupled with Fluoresbrite ® g at 25 °C. The pellet was resuspended in 400 μL of PolyLink coupling buffer ... highlights for children magazine historyWebApr 10, 2024 · The standard covalent coupling of the carboxylate-modified PS with carrier protein was conducted by using PolyLink Protein Coupling Kit (Cat. No. PL01N, Bang Laboratory Inc., USA) together with Vivaspin ultrafiltration device (Cat. No. AA022. small plate castersWebJul 1, 2009 · Coupling IgG to microbeads for affinity purificationRabbit IgGs (Sigma) are coupled to Carboxyl (COOH) microbeads (1 micron, Polysciences, Inc.) with the PolyLink Protein Coupling Kit for COOH microspheres (Polysciences) generally following the manufacturer’s instructions. small plate dinner serviceWebThe kit contents are sufficient for 50 coupling reactions using 1µm+ polymer (or magnetic) microspheres and 200-500µg of protein per reaction. Water Soluble Chemistry When the … small plate conceptWebPolyLink Protein Coupling Kit for COOH Microspheres PolyLink Protein Coupling Kit for COOH Microspheres Product Data Sheet 644 9025 Technology Dr. • Fishers, IN 46038-2886 800.387.0672 • 317.570.7020 • Fax 317.570.7034 [email protected] • www.bangslabs.com B E A D S A B O V E Description Carboxyl (COOH) microparticles can be used for covalent … highlights for children magazine reviewsWebprocedure of the PolyLink Protein Coupling Kit for COOH Microparticles (PolyScience, #24350) to covalently fix the beads and antibodies. ... Then, 0.17 mL of PolyLink coupling buffer included in the kit and 0.2 mL of goat anti-mouse IgG (H+L) cross-adsorbed secondary antibody were added to each tube and the beads were suspended using voltex. highlights for children my account