Phospho-ampk substrate motif

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August undefined, 2024

WebJan 10, 2024 · Further analysis using an AMPK consensus phosphorylation motif indicated that 32 of these sites are likely direct AMPK phosphorylation sites. We validated one uncharacterized protein, ARMC10, and demonstrated that the S45 site of ARMC10 can be phosphorylated by AMPK both in vitro and in vivo. WebJan 10, 2024 · We found that the phospho-AMPK substrate motif antibody, which is more specific than the phospho-Ser antibody, was able to detect MFF phosphorylation. However, the phospho-AMPK substrate... We would like to show you a description here but the site won’t allow us.

AMPK/ULK1-mediated phosphorylation of Parkin ACT domain

WebTable: PTMScan®Results,Phospho-AMPK Substrate Motif (LXRXXS*/T*), SILAC Study design: Human non-small cell lung cancer (H1650) cell line; LysC/post-IAP trypsin digest; … WebApr 11, 2024 · In LNCaP cells, inhibition of GSK3β by LiCl led to increased phosphorylation of its substrate glycogen synthase (pGS ... TPD52 characteristic D2-motif, PEST sequences, ... The results showed that the AICAR an activator of AMPK by phosphorylation significantly downregulated TPD52 expression in both LNCaP and VCaP cells. While investigating ... hikari langenhagen speisekarte

PTMScan® Phospho-AMPK Substrate Motif …

WebJul 9, 2024 · a p-AMPK substrate motif-specific antibody showing phosphorylation of GST-RUNX2-WT but not in lanes of serine-specific mutant of RUNX2-S118A, and GST control and b confirmed by [γ- 32 P]... WebNov 14, 2024 · Here, we provide evidence that PLIN3 is a novel and direct substrate of AMPK and PLIN3 phosphorylation by AMPK promotes LD dispersion, which implicates PLIN3 in lipolysis pathways. PLIN3 and PLIN2 are less efficient than PLIN1 at inhibiting basal lipolysis (Patel et al., 2014; Sanders et al., 2015 ). WebAug 12, 2024 · The C-terminal domain contains multiple protein kinase A (PKA) phosphorylation sites and is highly conserved between SIK1 and SIK2. 22 Like other AMPK family members, an activation loop (T-loop ... hikari led h11 bulbs

Phospho-AMPK Substrate Motif [LXRXX(pS/pT) MultiMab

WebNov 3, 2015 · To characterize the AMPK network, we conducted a screen to identify direct AMPK substrates and phosphorylation sites. We used an analog-specific (AS) method of kinase-substrate identification (Banko et al., 2011, Shah et al., 1997), which utilizes a mutant of AMPK that accepts N 6-(phenethyl) ATPγS, a bulky form of ATP introduced into cells … WebAnalysis of phospho-AMPK substrate motif antibody immunoprecipitates by MS/MS. (A, B) Primary hepatocytes were isolated from male C57BL/6 mice (12-week-old). Hepatocytes … ez paving fulton nyWebMar 18, 2024 · Although the phosphorylation motif is not a perfect fit, it has been previously shown that AMPK can potently phosphorylate its substrate without the canonical motif, such as ULK1 [Citation 63–65]. Importantly, while WT GST-TFEB is efficiently phosphorylated by AMPK in vitro , a truncation of the 36 C-terminal residues led to a dramatic ... ez pave barbados

"WebAMP-activated protein kinase (AMPK) is a conserved energy sensor that plays roles in diverse biological processes via phosphorylating various substrates. Emerging studies … " - Phospho-ampk substrate motif

Phospho-ampk substrate motif

WebJan 10, 2024 · The 109 phosphosites phosphorylated at higher levels in WT AMPK than in AMPKα1/α2-DKO cells were separated into groups 1 (with the conserved AMPK substrate motif) and 2 (without the motif). Webrecognizes other -3 arginine-bearing phospho-Ser/Thr peptides, such as substrate motifs for Akt and PKC, to a lesser extent. It does not recognize the nonphosphorylated substrate motif peptides. Phospho-(Ser/Thr) AMPK Substrate (P-S/T2-102) Rabbit mAb preferentially recognizes endogenous proteins and peptides bearing the LXRXXpS/pT motif.

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WebMay 1, 2015 · Here, we report an affinity proteomicstrategy for the discovery and validation of AMPK targets using an antibody to isolate proteins containing the phospho-AMPK substrate recognition motif from hepatocytes that had been treated with pharmacological AMPK activators. WebPTMScan® Phospho-AMPK Substrate Motif (LXRXXS*/T*) Kit Description: PTMScan® Technology employs a propri-etary methodology from Cell Signaling Technology for …

WebJan 10, 2024 · The phosphosites that were upregulated in WT cells but did not meet these rules and did not have sequences similar to the conserved AMPK substrate motif constituted group 2 ( n = 77). These sites may be regulated by the AMPK-dependent signaling pathways but are not directly phosphorylated by AMPK. WebIP. Immunoprecipitation of extracts from H1650 cells using Phospho-AMPK Substrate Motif [LXRXX (pS/pT) MultiMab ™ Rabbit mAb mix. Lane 1 shows 10% input. Western blot analysis was performed using the same antibody. Western blot analysis of extracts from various mouse tissues using Phospho-AMPK Substrate Motif [LXRXX (pS/pT) MultiMab ...

WebFeb 15, 2011 · To date, people have gathered large amounts of phosphorylation data, making it possible to perform substrate-driven motif discovery using data mining techniques. Results: We describe an algorithm called Motif-All that is able to efficiently identify all statistically significant motifs. The proposed method explores a support …

WebTo predict potential AMPK substrates, a sequence window of −5 to +4 positions around each phosphorylation site was scored with the AMPK motif analyzer 27. Putative AMPK phosphorylation sites ...

WebFeb 16, 2024 · A previous quantitative phospho-proteomics study uncovered an overall comprehensive regulatory role of AMPK during DDR with multiple newly identified substrates (Chen et al., 2024), while the intact role and related mechanism of AMPK in DSBR remained unknown.TALEN (transcription activator-like effector nuclease) technology was … hikari lyrics utadaWebAnalysis of peptides from H1650 cells gave 187 non-redundant sites containing AMPK substrate and related motifs. The primary motifs are highlighted in white. 83% of these sites contain phosphoserine, and the … ez pawn alvinWebPhospho-AMPK Substrate Motif [LXRXX (pS/pT) MultiMab ™ Rabbit mAb mix. Western blot was imaged using Odyssey ® Infrared Imaging System … hikari logabandonedWebDec 23, 2010 · Many in vivo substrates of AMPK not only conform to this motif but also bind to the phospho-binding protein 14-3-3 inducibly upon phosphorylation by AMPK. We therefore screened for proteins that bound to recombinant 14-3-3 in wild-type (WT) but not AMPK-deficient cells, and only under conditions of energy stress, when AMPK would be … hikari man animeWebAug 1, 2024 · Phosphorylation signal (as measured by pan phospho-AMPK-substrate antibody) was detected on HDAC9 when incubated with activated-AMPKα2β2γ2 complex … hikari man rawWebAnalysis of phospho-AMPK substrate motif antibody immunoprecipitates by MS/MS. (A, B) Primary hepatocytes were isolated from male C57BL/6 mice (12-week-old). Hepatocytes were incubated with ... hikari main taunus zentrumWebAMPK is a highly conserved sensor of intracellular adenosine nucleotide levels that is activated when even modest decreases in ATP production result in relative increases in … hikari lunch special