Phospho ampk antibody cst

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August undefined, 2024

WebAnti-AMPK alpha 1 (phospho T183) + AMPK alpha 2 (phospho T172) antibody [EPR5683] (ab133448) Research with confidence – consistent and reproducible results with every … WebApr 11, 2024 · We first found that phospho-AMPK signal widely overlapped with ... Samples were then incubated with 1:200 primary phosphorylated AMPK (pAMPK) antibody in 1.5% goat serum and 1% BSA in PBST at 4°C overnight. ... pERK (1:1000 for Western blotting and 1:2000 for IHC; Cell Signaling Technology, 4370S, lot 12, rabbit), tERK (1:1000; Cell …

AMPK and ACC Antibody Sampler Kit - Cell Signaling …

WebThe Phospho-AMPKalpha (Thr172) Antibody from Cell Signaling Technology is a Rabbit Polyclonal antibody to PRKAA1, and PRKAA2. This antibody recognizes Human, Mouse, Rat, and Non-Human Primate antigen. The Phospho-AMPKalpha (Thr172) Antibody has been validated for the following applications: Western Blot. WebSep 4, 2014 · Western blot analysis using a phospho-specific antibody revealed a significant increase in phospho-Thr184-AMPK levels in head tissue lysates from ElavGS>UAS-AMPK flies upon RU486 ... Primary antibody, rabbit anti-DILP2 (a generous gift from Dr. Seung Kim), or anti-GFP (Cell Signaling Technology; D5.1, XP) was added 1:250 and incubated … bishop sycamore clothing

AMPK/pAMPK Western Blot Analysis - Johns Hopkins …

WebAMPK is a heterotrimer consisting of a catalytic α-subunit and two regulatory subunits, β and γ. Each subunit exists as multiple isoforms (α 1, α 2, β 1, β 2, γ 1, γ 2, and γ 3 ), giving 12 different possible combinations of holoenzyme with different tissue distribution and subcellular localization ( 2 , 3 ). WebPhospho-AMPKalpha (Thr172) Antibody detects endogenous AMPKα only when phosphorylated at threonine 172. The antibody detects both α1 and … WebWestern blot analysis of Phospho-IRE1 alpha (Ser724) in HeLa cells. Samples were incubated in Phospho-IRE1 alpha (Ser724) polyclonal antibody (Product # PA1-16927) using a dilution of 2.0 µg/mL followed by an anti-rabbit HRP secondary antibody. Treated (+) or untreated (-) with 10 mM DTT for 60 min to activate the UPR. bishop sycamore coach roy johnson

Phospho-AMPK alpha-1,2 (Thr183, Thr172) Polyclonal Antibody

AMPK and ACC Antibody Sampler Kit - Cell Signaling …

Webantibody microarray Protein Expression & Phosphorylation Profiling Learn More Cell Signaling Phospho Antibody Array 16 Signaling Pathways PI3K/AKT AMPK Apoptosis Autophagy Cell Cycle ErbB Focal Adhesion … WebAll lanes : Anti-AMPK alpha 1 (phospho S487) antibody (ab131357) at 1/500 dilution Lane 1 : HUVEC cell extract untreated Lane 2 : HUVEC cell extract treated with UV Predicted band size: 64 kDa. Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AMPK alpha 1 (phospho S487) antibody (ab131357) bishop sycamore espn gameWebProtocol Outline. Prepare all reagents and samples as instructed in the manual. Add 100 µl of sample or positive control to each well. Incubate 2.5 h at RT or O/N at 4 °C. Add 100 µl of prepared primary antibody to each well. Incubate 1 h at RT. Add 100 µl of prepared 1X HRP-Streptavidin to each well. bishop sycamore college

"Web8 rows · Anti-phospho-AMPK α (Thr172) Antibody is an antibody against phospho-AMPK α (Thr172) for use in WB. Anti-phospho-AMPK α (Thr172) Antibody MSDS (material safety … " - Phospho ampk antibody cst

Phospho ampk antibody cst

AMPK antibody antibody review based on formal publications

Webphospho ampk alpha thr172 antibody ( Cell Signaling Technology Inc ) Cell Signaling Technology Inc is a verified supplier Cell Signaling Technology Inc manufactures this product About Bioz vStars Images anti ampk phospho t172 ( Cell Signaling Technology Inc ) Cell Signaling Technology Inc manufactures this product About News Press Release Team Webetary methodology from Cell Signaling Technology for peptide enrichment by immunoprecipitation using a specific bead-conjugated antibody in conjunction with liquid

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WebThe primary antibody to LC3B (ab192890, Abcam, Cambridge, MA, UK) or Cleaved Caspase 3 (#9664, CST, MA, USA) was added dropwise to the sections and incubated overnight at 4 °C. Next day, slices were washed three times in PBS before incubation with secondary antibody (A0208, Bain-marie Biotech, Shanghai, China) for 1 h at room temperature. WebFeb 26, 2024 · home > human > AMPK antibody This is a Validated Antibody Database (VAD) review about human AMPK, based on 7 published articles ( read how Labome selects the articles ), using AMPK antibody in all methods. It is aimed to help Labome visitors find the most suited AMPK antibody.

WebAMP-activated protein kinase (AMPK) and mechanistic target of rapamycin complex 1 (mTORC1) are meta- bolic kinases that co-ordinate nutrient supply with cell growth. AMPK negatively regulates mTORC1, and mTORC1 reciprocally phosphorylates S345/7 in both AMPK a-isoforms. WebPhospho-AMPKα (Thr172) (40H9) Rabbit mAb detects endogenous AMPKα1 only when phosphorylated at threonine 183 and endogenous …

Web使用 Phospho-AMPKα (Thr172) (40H9) Rabbit mAb（上）或 AMPKα Antibody #2532（下），对未经处理或经寡霉素处理 (0.5 µM) 的 C2C12 细胞提取物进行蛋白质印迹分析。使用 Phospho-AMPKα (T172) (40H9) Rabbit mAb，对对照（左）或经苯乙双胍处理（右）的石蜡包埋的 NCI-H228 细胞沉淀物进行免疫组织化学分析。使用 Phospho-AMPKα (Thr172) … WebCell Signaling Abs (4116) » ErbB Signaling Pathway (725) By Disease » Viral Myocarditis Ab (351) By Biomarker » Cancer Biomarkers (4587) ... (phospho Y393/429) polyclonal antibody (Cat # PAB12190). Immunofluorescence; Immunofluorescence staining of methanol-fixed HeLa cells using ABL1/ABL2 (phospho Y393/429) polyclonal antibody (Cat ...

WebThe Human Phospho-Kinase Array (Catalog # ARY003B) is a rapid, sensitive, and economical tool to simultaneously detect the relative site-specific phosphorylation of 43 kinases and 2 related total proteins without performing …

WebPhospho-AMPK alpha-1,2 (Thr183, Thr172) Recombinant Polyclonal Antibody (10HCLC) Advanced Verification This Antibody was verified by Cell treatment to ensure that the antibody binds to the antigen stated. View all (16) AMPK alpha-1,2 antibodies Datasheet Protocols Questions & Answers Tech Support Antibody Testing Data (4) Advanced … dark souls old witch\\u0027s ringWebMonoclonal Antibody for studying ERK1 (Thr202/Tyr204) phosphate/ERK2 (Thr185/Tyr187) phosphate. Cited in 950 publications. Validated for WB, IP, IHC. Highly specific and rigorously validated in-house, Phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) (20G11) Rabbit Monoclonal Antibody (CST #4376) is ready to ship. bishop sycamore football coaching staffWebAnti-phospho-AMPK α (Thr172) Antibody is an antibody against phospho-AMPK α (Thr172) for use in WB. Anti-phospho-AMPK α (Thr172) Antibody MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and … bishop sycamore football newsWeb® 2015 Cell Signaling Technology, Inc. Orders ff 77-1-CELL (255) orderscellsignal.com Support 77-7-TECH (2) infocellsignal.com Web ff www.cellsignal.com page 2 of 2 9957 For western blots, incubate membrane with diluted primary antibody in either 5% w/v BSA or nonfat dry milk, 1X TBS, 0.1% Tween® 20 at 4°C with gentle shaking, overnight. bishop sycamore football columbus ohioWebPhospho-(Ser/Thr) AMPK Substrate (P-S/T2-102) Rabbit mAb preferentially recognizes endogenous proteins and peptides bearing the LXRXXpS/pT motif. The antibody also … bishop sycamore footballWebAMP-activated protein kinase (AMPK) is a key cellular energy sensor and regulator of metabolic homeostasis. Although it is best known for its effects on carbohydrate and lipid … bishop sycamore football head coachWebMonoclonal Antibody for studying Bad (Ser99) phosphate. Cited in 1 publication. Highly specific and rigorously validated in-house, Phospho-Bad (Ser136) (5D8) Mouse … dark souls old witch\u0027s ring effect