WebThe Phospho-Akt (Thr308) Antibody was generated using AKT1, AKT2, and AKT3 as the antigen and it reacts with Human, Mouse, and Rat. Phospho-Akt (Thr308) Antibody detects endogenous levels of Akt only when phosphorylated at Thr308 Phospho-Akt (Thr308) Antibody Supplier Page Product Specs Company Info Citations Reviews Product Specs Webwavelength (665 nm). The specific signal modulates positively in proportion to phospho-AKT (Thr308). The assay can be run under a two-plate assay protocol, where cells are plated, stimulated and lysed in the same culture plate. Lysates are then transferred to the assay plate for the detection of phospho-AKT (Thr308) by HTRF ® reagents.
Anti-phospho-Akt (Thr308) Antibody 07-1398 - EMD Millipore
WebAkt/PKB growth Ser241 Thr308 Ser473 Akt/PKB Thr308 Ser473 glycogen synthesis proliferation survival PI3K Akt, Akt Section 03-02-06 PIP 3 PIP 3 Tyr376 Tyr373 SHIP 05_PW_Akt/PKB.eps Tyr326 Src M e m b r a n e PDK1 P P P P P P P P P P P P P P P P mTOR SIN1 GβL Rictor Akt-PKB KSignaling Pathway Phospho-Akt (Ser473) kDa 105 76 57 46.5 ... WebAkt is partially activated by phosphorylation of T308 by PDK1. Full activation requires phosphorylation of S473, which can be catalysed by multiple proteins, including phosphoinositide-dependent kinase 2 (PDK2), integrin-linked kinase (ILK), [1] mechanistic target of rapamycin complex complex 2 (mTORC2) and DNA-dependent protein kinase … birla clothing brands
Phospho-AKT1/AKT2/AKT3 (Thr308) Polyclonal Antibody
WebMay 24, 2011 · The phosphorylation of Thr308 is a more reliable biomarker for the protein kinase activity of Akt in tumour samples than Ser473. Any evaluation of the link between … WebMar 22, 2024 · The protein levels of p-Akt (S473 and T308), Akt, p-rpS6 (S235/236, S240/244), rpS6, FLAG and β-actin were detected with immunoblotting. e HEK293T cells were transfected with FLAG empty vector or FLAG-DRAM1 for 24 h, and then starved for another 18 h before stimulated with IGF-1, EGF or serum (10%) for 10 min in the presence … WebAKT also known as protein kinase B (PKB) or RAS-alpha, is an ubiquitous serine/threonine kinase that plays an important role in diverse biological responses such as regulation of metabolism, cell survival and growth by phosphorylating multiple proteins. birla corporation annual report