Phis2 酵母单杂

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August undefined, 2024

Webb1 mars 2024 · VvACTIN or AtACTIN2 was used as internal references for grape and Arabidopsis. The qRT-PCR primers are listed in Table 1. Table 1. Gene-specific primers used for quantitative real-time PCR assays. 2.7. Transformation of Arabidopsis The 35S: VvWRKY30 vector was created by cloning genes into the pSuper1300 binary vector with … http://www.biofeng.com/zaiti/jiaomuzajiao/pHis2.html

酵母单杂交详细实验流程-钟鼎生物

Webb1 dec. 2024 · AD/pATM1-pHIS2 and AD-ATM1/pATM1-pHIS2 were cotransformed into the Y187 strain and cultured on SD/-Trp/-Leu for 2–3 days at 30 °C. Then, healthy and consistent clones were diluted, ... http://www.coolaber.com/usr/2160/File/F/F4671.pdf chine meat

Y187-pHis2系统酵母单杂培养基套装说明书-分析方法-生物在线 …

Webb27 mars 2024 · 筛选含有报道质粒的酵母细胞具体过程为：合成靶序列；将靶序列插入pHIS2载体的多克隆位点；一般为将0.1mg pHIS2质粒用EcoRⅠ和SacⅠ进行完全双酶 … Webb1 juni 2024 · Clone the products of the PCR reactions carried out in step 3 into the pHIS2 T-vector obtained in step 5. The T-A ligation system includes 0.2 μg of T-vector of pHIS2, 0.5 μL of PCR products (Y1 + Y3 and Y2 + Y3), 1 μL of 10× ligation buffer, 3 U of T4 ligase, and PEG6000 at final concentration of 10% (w/v). Webb22 juli 2013 · 酵母单杂交系统 Clontech pHis2说明书 . Description. pHIS2 is a reporter vector that can be used in yeast one-hybrid assays to identify and characterize DNA … grand canyon where to fly

请教一个酵母单杂交的问题 - 分子生物 - 生物化学 - 小木虫论坛-学 …

Webb质粒名称：pHis2 出品公司：Clontech 质粒类型：酵母单杂交系统克隆方法：多克隆位点，限制性内切酶载体大小： 7207bp 原核抗性： Kanamycin (卡那霉素) 真核抗性：TRP1 (HIS3 selection) 备注： Clone in cis-acting sequences for use with 1-hybrid system 载体介绍 pHIS2 is a reporter vector that can be used in yeast one-hybrid assays to identify … http://www.bioon.com.cn/doc/showarticle.asp?newsid=96627 chine moveWebb6 sep. 2024 · 摘要：本文针对酵母单杂技术的基本原理、基础知识、主要用途做了简要介绍；并以Y1Hgold（pAbAi-bait）和Y187（pHis2-bait）为例，介绍了自激活检测的目的以 … chinemys megalocephala

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Phis2 酵母单杂

WebbpHIS2 Vector Information PT3705-5 Sold as part of Catalog #K1617-1 Restriction Map and Multiple Cloning Site (MCS) of pHIS2 Vector. Unique restriction sites are in bold. *Note: The Sac II site is resistant to cleavage. Description pHIS2 is a reporter vector that can be used in yeast one-hybrid assays to identify and characterize DNA-binding ... Webb本文针对酵母单杂技术的基本原理、基础知识、主要用途做了简要介绍；并以Y1Hgold（pAbAi-bait）和Y187（pHis2-bait）为例，介绍了自激活检测的目的以及主要 …

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http://www.bioon.com.cn/reagent/show_product.asp?id=3900880 WebbpHis2 价格： 1000元联系方式： I47-825O-882O 立刻购买买家导航载体基本信息载体质粒图谱和多克隆位点信息载体简介载体序列 LOCUS pHIS-2 7207 bp DNA SYN DEFINITION pHIS-2 ACCESSION KEYWORDS SOURCE ORGANISM …

WebbpHIS2 PVT4037 2ug Lot no 1901093 Store at -20℃ Expiry date June.2024 pHIS2 Information Promoters: TRP1, T7, T3 Replicon: pUC ori, FI ori Plasmid classification: yeast series, yeast one hybrid vector Plasmid size: 7207bp … WebbPhis2osgpx1 Promoter Phis2 1, supplied by TaKaRa, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more. Home > Search Results > TaKaRa > phis2 1. phis2 1 vector TaKaRa is a verified supplier TaKaRa ...

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WebbpHis2酵母单杂交载体是由Biovector Co.,LTD代理或销售的Biovector, Addgene, ATCC, Invi品牌的试剂，产品来源于Biovector Inc. USA。Biovector Co.,LTD是中国最权威的pHis2酵母单杂交载体试剂销售服务商之一，在海淀区等地方销售pHis2酵母单杂交载体试剂已经多年。生物在线为您提供众多企业pHis2酵母单杂交载体仪器产品及 ...

http://www.honorgene.com/product_list/carrier_library/123112.html grand canyon where to goWebb产品名称：Y187-pHis2 Yeast One-Hybrid Media Kit Y187-pHis2 酵母单杂培养基套装（ZC1902）产品介绍说明书下载产品介绍：酵母杂交培养基套装包含酵母杂交文库筛 … chine meansWebb1 apr. 2024 · The Y187 strain was prepared for the Y1H assay, we mixed pAD-CmBBX7 and pAD-GUS with pHIS2-CmFTL1pro respectively, and transformed them into Y187 strain using the lithium acetate method.Then inoculated on SD/His-Trp-Leu-plates, and screened for the lowest concentration range of 3-AT that was sufficient to inhibit the growth of … chi-ne-myonplanhealthWebb酵母单杂交体系是在酵母双杂交体系基本原理的基础上，通过观察酵母细胞内报告基因的表达状况，在酵母细胞内研究DNA与蛋白质之间的相互作用的一种技术。许多真核生物的 … chin emgWebb12 apr. 2024 · 目的：筛选与启动子pHIS2-A互作的蛋白。方法：以启动子A构建到酵母单杂交启动子载体pHIS2，文库质粒为pGADT7-中山杉文库。材料： 1．启动子克隆：pHIS2-A。根据实验需求进行扩增并酶切构建载体，两端的EcoRI和 SacI酶切位点，序列如下： GAATTCAGTTGGAATCACATCTATCATCAGAATGAAAGACCTAAGAAAAAAATGGCA … chine moheWebb人机验证 chi ne my online healthWebb22 juli 2013 · 酵母单杂交系统 Clontech pHis2说明书 Description pHIS2 is a reporter vector that can be used in yeast one-hybrid assays to identify and characterize DNA-binding proteins. The vector was specifically designed for use with the BD Matchmaker One-Hybrid Library Construction & Screening Kit （#K1617-1）. grand canyon whitewater