WebMar 16, 2024 · After antibody incubation, some brain sections were stained with FluoroMyelin Green Fluorescent Myelin Stain (1:300; Invitrogen) for 20 min at room temperature. For samples with a determined number of total cells, their nuclei were counterstained for 5 min with 100 n M 4, 6-diamidino-2-phenylindole dihydrochloride … WebJan 4, 2024 · The sections were incubated with FluoroMyelin Green fluorescent myelin stain (1:300, Molecular probes) for 20 minutes at room temperature. Fluoromyelin staining images were viewed on a Nikon upright microscope, and the images from fluoromyelin stains were analyzed using ImageJ.
Autofluorescence enhancement for label-free imaging of …
WebThe FluoroMyelin Green fluorescent myelin stain enables quick and selective labeling of myelin in brain cryosections in a single 20-minute labeling step plus washes. This stain … WebJan 2, 2013 · Myelins were visualized using FluoroMyelin green fluorescent myelin stain kit (Molecular probes). IgG staining. After PFA fixation, 12-μm–thick brain sections were incubated in 3% H 2 O 2, followed by blocking with 10% bovine serum albumin (Sigma-Aldrich) in PBS. chinese takeout bethesda
Invitrogen FluoroMyelin Red Fluorescent Myelin Stain - Solution in ...
WebAfter fixation, frozen sections (spinal cord) were incubated with FluoroMyelin™ green fluorescent myelin stain according to the manufacturer’s instructions (Thermofisher Scientific) or with CD3 and CD4 antibodies (table S2) as previously published ( Larochelle et al., 2012, Larochelle et al., 2015, Larochelle et al., 2024 ). WebNov 9, 2024 · To further validate the ability of myelin labeling in the CNS by PM-ML, the cryosection of corpus callosum (CC), which are rich in myelinated nerve fibers, or CB … WebApr 20, 2024 · Spinal cord sections were stained with 4′,6-diamidino-2-phenylindole (Vector) and Fluoromyelin green fluorescent myelin stain (ThermoFisher) following the manufacturer’s instruction. Hematoxylin and eosin staining was performed following standard protocol as previously described. 17 Flow cytometry grandview veterinary clinic